Kisspeptin – 10mg

Kisspeptin-10 – Neuroendocrine Peptide for Reproductive & HPG Axis Research Kisspeptin-10 (KP-10, Metastin 45-54) is a 10-amino acid decapeptide representing the minimal biologically active sequence of the kisspeptin family. Derived from the C-terminal region of the 145-amino acid KISS1 gene precursor protein, Kisspeptin-10 retains full receptor binding affinity at the GPR54 (KISS1R) receptor and has been extensively studied for its role in hypothalamic-pituitary-gonadal (HPG) axis regulation in research settings. As a research peptide, Kisspeptin-10 has been investigated for GnRH neuron stimulation, gonadotropin secretion mechanisms, reproductive neuroendocrinology, puberty onset signaling, and anti-metastatic activity in controlled laboratory and preclinical environments. At a Glance Classification: Endogenous neuropeptide / RFamide family member Amino Acid Length: 10 amino acids (C-terminal amidated decapeptide) Molecular Weight: ~1,302.45 Da (1.30 kDa) Purity: ≥99% (HPLC verified) Primary Research Areas: HPG axis regulation, GnRH stimulation, reproductive neuroendocrinology Storage: -20°C (lyophilized powder), 2-8°C (reconstituted) GnRH Stimulation Research Investigated for potent stimulation of gonadotropin-releasing hormone secretion from hypothalamic neurons in research models Gonadotropin Secretion Studies Research explored downstream LH and FSH release mechanisms through HPG axis activation in laboratory models Reproductive Axis Investigations Investigated for hypothalamic-pituitary-gonadal axis regulation and neuroendocrine feedback mechanisms GPR54 Receptor Signaling Studies explored KISS1R binding, PIP2 hydrolysis, Ca²⁺ mobilization, and ERK1/2 phosphorylation pathways Puberty Onset Research Investigated for role in initiating pubertal GnRH secretion and developmental timing mechanisms in research Anti-Metastatic Studies Research on KISS1-derived peptide activity in metastasis suppression and cell migration inhibition models Kisspeptin-10 represents the minimal active fragment of the kisspeptin neuropeptide family, investigated for potent GPR54 receptor activation, GnRH neuron stimulation, gonadotropin release, and reproductive axis regulation in controlled research environments. This peptide is supplied strictly for laboratory research purposes only. Not intended for human or animal consumption. Not FDA-approved for any medical use. Scientific Overview of Kisspeptin-10 Kisspeptin-10 is a 10-amino acid decapeptide derived from the C-terminal region of kisspeptin-54 (formerly termed metastin), which is itself generated by proteolytic cleavage from a 145-amino acid precursor protein encoded by the KISS1 gene. Originally identified as a metastasis suppressor gene product in 1996, the reproductive significance of the kisspeptin system was discovered in 2003. Sequence: Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂ (YNWNSFGLRF-NH₂) C-terminal amidation: RFamide motif required for GPR54 receptor activation Minimal active fragment: Retains full receptor binding affinity comparable to kisspeptin-54 in vitro Kisspeptin-10 belongs to the RFamide peptide family, characterized by a conserved arginine-phenylalanine-NH₂ motif at the C-terminus. Research has demonstrated that this decapeptide sequence is sufficient to fully activate GPR54 (KISS1R), with binding affinity values of approximately 1.59 nM in rat and 2.33 nM in human receptor models. The KISS1 gene was first isolated in Hershey, Pennsylvania, and named for its location near the Hershey’s chocolate factory. Its role as a critical upstream regulator of the reproductive endocrine axis was revealed when inactivating mutations in GPR54 were linked to reproductive dysfunction in research models. Mechanism of Action Mechanistically, Kisspeptin-10 has been investigated for effects through multiple neuroendocrine signaling pathways in research models: GPR54 (KISS1R) Receptor Activation: Research documented Kisspeptin-10 binding to the G protein-coupled receptor GPR54, triggering intracellular signaling cascades investigated for: PIP2 hydrolysis: Phospholipase C activation and inositol trisphosphate generation in cellular models Calcium mobilization: Intracellular Ca²⁺ release triggering downstream signaling in neuronal studies ERK1/2 phosphorylation: Extracellular signal-regulated kinase activation in KISS1R-expressing cells p38 MAPK signaling: Stress-activated protein kinase pathway engagement in receptor studies GnRH Neuron Stimulation: Studies explored Kisspeptin-10’s role as a direct activator of hypothalamic GnRH neurons investigated for: Pulsatile GnRH release: Stimulation of gonadotropin-releasing hormone secretion in neuroendocrine models LH surge induction: Luteinizing hormone release through anterior pituitary stimulation in research FSH secretion: Follicle-stimulating hormone release mechanisms in gonadotropin studies HPG axis activation: Full reproductive axis engagement from hypothalamic level in preclinical models KNDy Neuron Network Integration: Research documented Kisspeptin’s role within the KNDy (Kisspeptin, Neurokinin B, Dynorphin) neuronal network investigated for: Endocrine feedback integration from gonadal steroid signals in regulatory studies Metabolic status sensing and energy balance–reproduction coupling in research models Pulsatile GnRH secretion pattern generation in neuroendocrine investigations Thermoregulatory center interactions in hypothalamic pathway studies Anti-Metastatic Signaling: The original KISS1 gene discovery involved metastasis suppression, with research documenting: Melanoma and breast cancer cell migration inhibition in in-vitro models Trophoblast cell migration regulation in placental invasion studies Cell invasion suppression pathways in tumor biology research Metastasis suppressor gene activity in cancer cell line investigations Pharmacology & Pharmacokinetics Research has characterized Kisspeptin-10’s pharmacological properties through receptor binding assays, in-vitro signaling studies, and in-vivo neuroendocrine models. Pharmacological Characteristics Investigated: Receptor Affinity: Ki values of ~1.59 nM (rat) and ~2.33 nM (human) at GPR54 in binding studies Circulating Half-Life: Approximately 6-fold shorter than kisspeptin-54 in pharmacokinetic research In-Vitro Potency: Comparable receptor activation to full-length kisspeptin-54 in cellular assays Critical Residues: Phenylalanine at positions 6 and 10 essential for KISS1R binding in structure-activity studies Helical Structure: Alpha/3₁₀-helix between residues 4–10 documented by NMR in conformational research These pharmacokinetic properties position Kisspeptin-10 as a research tool investigated for acute neuroendocrine stimulation studies, receptor pharmacology experiments, and reproductive axis activation research requiring defined GPR54 agonist activity. Molecular Specifications: Molecular Formula: C₆₃H₈₃N₁₇O₁₄ Molecular Weight: ~1,302.45 Da (1.30 kDa) CAS Number: 374675-21-5 Appearance: White lyophilized powder Solubility: Water-soluble when properly reconstituted Research Findings & Applications Preclinical and translational research involving Kisspeptin-10 has explored multiple neuroendocrine and reproductive systems: Research Applications Investigated: Reproductive axis activation: GnRH stimulation and gonadotropin release mechanisms in neuroendocrine models Puberty onset research: Developmental timing and pubertal transition signaling in preclinical studies Fertility investigation models: Ovulation stimulation and HPG axis dysfunction research in laboratory settings Hypogonadotropic hypogonadism: Diagnostic differentiation and reproductive axis assessment in research Metastasis suppression studies: Cell migration inhibition and tumor spreading prevention in cancer models Metabolic-reproductive coupling: Energy balance and fertility signaling integration in neuroendocrine research The potent GPR54 agonist activity and minimal peptide length make Kisspeptin-10 a widely used research tool in reproductive neuroendocrinology, offering cost-effective receptor activation studies compared to full-length kisspeptin-54. Key Research Observations: Research across multiple experimental settings has documented: Potent and rapid LH secretory responses following administration in preclinical models Full KISS1R activation with binding affinity comparable to longer kisspeptin isoforms in vitro Anti-metastatic activity in melanoma and breast cancer cell migration assays Trophoblast migration regulation relevant to placental biology research Safety Profile in Research Context Kisspeptin-10 is intended exclusively for laboratory research use in controlled experimental environments. Research Safety Observations: Well-characterized receptor pharmacology with defined binding kinetics in research models Structure-activity relationships extensively mapped through systematic analog studies Short circulating half-life documented in pharmacokinetic research Safety profiles documented in preclinical neuroendocrine research settings only No claims are made regarding safety, efficacy, or suitability for human or animal use. All research findings apply strictly to controlled laboratory investigations. Frequently Asked Questions What is Kisspeptin-10 and how does it relate to the kisspeptin family? Kisspeptin-10 (KP-10) is a 10-amino acid decapeptide representing the minimal biologically active sequence of the kisspeptin peptide family. It shares its C-terminal sequence with the larger isoforms—kisspeptin-54, kisspeptin-14, and kisspeptin-13—all derived from a 145-amino acid precursor encoded by the KISS1 gene. Despite being the smallest active fragment, KP-10 retains full receptor binding affinity at GPR54 (KISS1R) comparable to the full-length kisspeptin-54 in vitro. What research areas has Kisspeptin-10 been investigated for? Research has explored Kisspeptin-10 in reproductive neuroendocrinology, GnRH neuron stimulation, gonadotropin (LH/FSH) secretion studies, puberty onset mechanisms, HPG axis regulation, fertility and ovulation research, hypogonadotropic hypogonadism diagnostic models, anti-metastatic studies in cancer biology, and metabolic-reproductive coupling investigations. What are the primary mechanisms of action documented in research? Research has documented Kisspeptin-10 as a potent GPR54 (KISS1R) agonist that triggers PIP2 hydrolysis, intracellular calcium mobilization, ERK1/2 and p38 MAPK phosphorylation, and arachidonic acid release. These intracellular events stimulate GnRH release from hypothalamic neurons, which drives downstream LH and FSH secretion through the anterior pituitary, activating the full hypothalamic-pituitary-gonadal reproductive axis. What is the molecular weight and purity of this compound? Kisspeptin-10 has a molecular weight of approximately 1,302.45 Da (1.30 kDa) with a molecular formula of C₆₃H₈₃N₁₇O₁₄ and a CAS number of 374675-21-5. Our product maintains ≥99% purity as verified by HPLC analysis, with each batch including a Certificate of Analysis confirming identity, purity, and quality through advanced analytical methods. How should this research peptide be stored? Store lyophilized powder at -20°C protected from light and moisture. Once reconstituted, store at 2-8°C and use within the timeframe specified in the reconstitution protocol. For long-term storage of reconstituted peptide, addition of a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles. Detailed storage and reconstitution specifications are included with Certificate of Analysis documentation. What was the safety profile observed in research? Kisspeptin-10 has been characterized through extensive receptor pharmacology and structure-activity relationship studies in preclinical research settings. Its short circulating half-life and well-defined binding kinetics at GPR54 have been documented in pharmacokinetic research. This compound is strictly for laboratory research purposes. How does Kisspeptin-10 compare to Kisspeptin-54? Kisspeptin-10 and Kisspeptin-54 share the same C-terminal decapeptide sequence and bind GPR54 with comparable affinity in vitro. However, KP-54 is more potent in vivo, likely due to its longer circulating half-life (approximately 6-fold greater than KP-10). KP-10 offers advantages as a research tool due to its smaller size, lower production cost, and well-defined structure-activity relationships that have been extensively mapped through systematic analog studies. Research Status & Applications Kisspeptin-10 remains a cornerstone research tool in reproductive neuroendocrinology, investigated for its role in GnRH neuron activation, gonadotropin secretion, HPG axis regulation, puberty onset mechanisms, and anti-metastatic signaling in controlled laboratory and preclinical environments. Current research interests focus on reproductive axis diagnostic applications, fertility-related neuroendocrine pathways, metabolic-reproductive integration mechanisms, and kisspeptin analog development for enhanced in-vivo bioactivity in experimental models. The potent GPR54 agonist activity and minimal active fragment characteristics of Kisspeptin-10 continue to make it an essential research tool across reproductive biology, neuroendocrinology, and cancer biology research disciplines. This peptide is not FDA-approved for any medical use and is supplied strictly for laboratory research purposes only. Not intended for human or animal consumption.